Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Live imaging of cell doublets
Request a Protocol
Ask a
question
Favorite
Dissociated cells labelled with GAP-Cherry or GAP-YFP were mixed and plated on glass bottom dish containing 1×MBS-H. Images were acquired using a Quorum technologies WaveFX spinning disc confocal mounted on an automated DMI6000B Leica microscope, with a 40x HCX PL APO CS, NA = 1.25 oil objective was used. Overall, 491 and 561 nm diode lasers were used for GFP and Cherry excitation, respectively. Images were collected with EM CCD 512X512 BT camera using the acquisition software Improvision Volocity 3DM.
Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
