Specificity and Sensitivity Evaluation

Clinical specimens that were confirmed and genotyped previously by Department of Microbiology, Zhejiang CDC, were used as positive controls for MP, HV, SFTSV, DENV, CHIKV, FluA, and FluB. The targeted genes of the remaining pathogens were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) and used for plasmid construction. Then, the RNAs were transcribed in vitro and also used as templates to assess the specificity of the microarray. The sensitivity of the microarray analysis was evaluated by serial 10-fold dilutions of in vitro-transcribed RNAs (ranging from 10⁵ to 10¹ copies/μl). Ebola virus (Zaire) Nucleic Acid Detection Kit (real-time PCR) (Puruikang Biotech Co., Ltd., Shenzhen, China) was also used as references for sensitivity evaluation.