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The dried resin was treated with TFA/H2O/TIPS (95:2.5:2.5 v/v/v), or TFA/H2O/DODT (95:2.5:2.5 v/v/v) (2–3 ml per 100 mg of resin) and shaken for 2 h a t 25°C. After filtration, the filtrate was concentrated by blowing with a gentle flow of N2. Add the precooled diethyl ether to precipitate crude peptides. The resulted suspension was centrifuged (8,000 rpm, 5 min, 4°C), and the ether layer was decanted. Air-dry the peptide product in the open centrifuge tube for about 30 min. The targeted crude peptide was obtained as the solid.
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