2.2. Hyperthermia‐induced seizures

To evaluate the temperature at which Scn1a^A1783V/WT mice seize, mice were placed under a heat lamp and core temperature was gradually raised in an acrylic glass chamber until a generalized seizure was observed or the temperature reached 42.5°C.^{18, 25, 26, 27} After the procedure, mice were transferred to a cool, granite block to rapidly lower the core temperature, and then they were returned to their home cages. Body temperature was monitored using a neonate rectal probe (Braintree Scientific, Inc, Braintree, MA) coupled to a TCAT‐2LV controller (Physitemp Instruments, Inc). Mice acclimated in the chamber for 5 minutes prior to experiments. Male and female Scn1a^A1783V/WT randomly received drug or vehicle prior to the test and staff were blinded to the treatment. If a mouse had a behavioral seizure between drug administration and testing, it was removed from the study. The temperature at which mice seized was recorded. In experiments where wild‐type mice were used, the staff was also blinded to genotype. Average baseline temperatures and the number of male and female mice used for each compound tested are shown in Table S1, with minimal variability between tests. The rate of temperature change (temperature (°C)/time to seizure (s)) for each drug tested is also shown in Table S2.