2.4. Transfection of primary neurons

The miR‐132 mimics and its control, miR‐132 inhibitors and its control, small interfering RNAs (siRNAs) targeting MMP‐9 and the negative control siRNA were designed and constructed by RiboBio. After 7 days culture in vitro, the primary neurons were transfected by using Transfection regent ({"type":"entrez-nucleotide","attrs":{"text":"C10511","term_id":"1535582","term_text":"C10511"}}C10511‐05 riboFECT CP Transfection Kit, RiboBio) according to the manufacturer's instructions. We used MMP‐9 siRNA and miR‐132 mimics at 50 nM and miR‐132 inhibitors at 100 nM final concentration. The MMP‐9 siRNA sequences were CCGGGAACGTATCTGGAAA. The expression of target genes after transfection of siRNA is about 70% according to our experience.