Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
RNA binding immunoprecipitation (RIP) assay
Ask a
question
Favorite
The Magna RIP RNA Binding Protein Immunoprecipitation kit (Product ID: RIP; MilliporeSigma) was used for RIP, according to the manufacturer's instructions. MKN45 cell lysates were collected and 100 µl lysate per reaction was incubated with NC IgG (1:500; cat. no. ab172730) or anti-EIF4A3 (1:1,000; cat. no. ab180573) antibody (both Abcam) conjugated to protein A/G magnetic beads (EMD Millipore) at 4°C overnight. Immunoprecipitated RNA was then extracted and detected by RT-qPCR to confirm the enrichment of binding targets.
Copyright and License information: ©2021 Wu et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
