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Measurement of neutrophil infiltration in the lungs
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A formalin-fixed left lower lung lobe tissue sample was prepared from each animal. Samples were dehydrated and embedded in Paraplast (Leica Microsystems GmbH, Wetzlar, Germany). The tissues were cut into 4-μm-thick sections and stained with H&E. Lung neutrophil infiltration was examined by a pathologist who was blinded to the group allocation under a light microscope.
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Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
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