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Transmission electron microscopy (TEM)

LW Lin-Ping Wu
MF Mario Ficker
JC Jørn B. Christensen
DS Dmitri Simberg
PT Panagiotis N. Trohopoulos
SM Seyed M. Moghimi
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A 10 μL of 0.05 mgmL−1 Pc-dendrimer complexes was placed onto a 200 mesh carbon-coated copper grids and allowed to stand for 5 min. Excess solvent was carefully removed by capillary action using a filter paper and the sample was immediately stained with 10 μL of 2% (v/v) phosphotungstic acid solution for 1 min. Excess stain was removed and the grids were allowed to dry for 30 min. Images were taken with a Philips CM100 transmission electron microscope (FEI/Philips, Eindhoven, Netherland) with an accelerating voltage of 80 kV. Image analyses were performed with Matlab 2014b (Mathworks, MA, USA).

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