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RNA-Fluorescence in situ Hybridization
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Hybridization of circGNG4 was carried out using a Cy2-labeled probe, and the miR-223 was carried out using a Cy5-labeled probe according to the manufacturer’s protocol (Shanghai GenePharma Co., Ltd., Shanghai, China). DAPI was added to stain the cell nucleus. The subcellular distribution of circGNG4 and miR-223 in prostate cancer cells was observed using a confocal laser scanning microscope (FV1000; Olympus Corporation, Japan).
Copyright and License information: ©2021 Xu, Lian, Zhang, Xu and Zhang.
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