Cell viability assay.

SK Shuichi Kimpara
LL Li Lu
NH Nguyet M. Hoang
FZ Fen Zhu
PB Paul D. Bates
AD Anusara Daenthanasanmak
SZ Shanxiang Zhang
DY David T. Yang
AK Amanda Kelm
YL Yunxia Liu
YL Yangguang Li
AR Alexander Rosiejka
AK Apoorv Kondapelli
SB Samantha Bebel
MC Madelyn Chen
TW Thomas A. Waldmann
CC Christian M. Capitini
LR Lixin Rui
request Request a Protocol
ask Ask a question
Favorite

Cell viability was measured with an automatic cell counter according to the manufacture’s instruction. Cells were harvested and suspended, and mixed with equal volume of 0.4% trypan blue in PBS. Ten μl of the cell suspension was loaded onto TC20 system (Bio-Rad) counting slides, and the number of viable cells was quantified on a TC20 automated cell counter (Bio-Rad).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A