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Wound-healing tests were used to measure cell migration. Stable PC3 and DU145 cells were seeded on 24-well plates. When 90–100% confluence was achieved, a 200-µl sterile pipette was used to create a linear scratch. After washing with a serum-free medium, the cells were cultured for 48 h. Cell images at 0 and 48 h after injury were recorded under a microscope.

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