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The stable PC3, DU145, and LNCaP cells were seeded into 96-well plates at the density of 5 × 103 cells/well. Cell Counting Kit-8 (CCK-8) (Bimake, USA) solution was added to each well to a final concentration of 0.5 mg/ml and incubated at 37 °C for 1 h. The absorbance was measured at 450 nm using a plate reader (Model 680; Bio-Rad Laboratories).
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