4.4.4. Immunofluorescence Microscopy to Evaluate the Activity of GLUT4 and mTOR Proteins

The harvested cells were examined by immunofluorescence staining with specific polyclonal antibodies against solute carrier family 2 member4 (SLC2A4/GLUT4) (cat. no. FNab03503, FineTest, Wuhan, China) and mammalian target of rapamycin (m-TOR) protein (cat. no. FNab05417, FineTest, Wuhan, China) (green) at dilutions 1:100. An Alexa Fluor 488 anti-rabbit IgG secondary antibody (cat. no. A-11034, Invitrogen; ThermoFisher Scientific, Hilden, Germany) was used for detection at dilution 1:100 in antibody dilution buffer. Fluorescence was examined by an immunofluorescence microscope LX400 (cat. no. 9126000, Labmed; USA), using Optika ISview image acquisition and processing software.