4.7. In Vitro Leishmanicidal Assay

The assays were performed according to the procedures described by Mikus and Steverding [79] with some adjustments. The activity of the oil was performed in triplicate. Promastigotes forms (2.5 × 10⁵ parasites/well) were cultured in 96-well plastic plates. The samples were dissolved in dimethylsulfoxide (DMSO). Different dilutions of the compounds, up to 200 mL of the final volume, were added. After 48 h at 26 °C, 20 µL of resazurin solution was added and the oxidation–reduction was measured from 570 to 595 nm. In each assay pentamidine was used as a control reference drug. The anti-promastigotes percentages (AP%) were calculated. The efficacy of each compound was determined.