2.5. Drug Release from Nanoparticles

CIP release from nanoparticles was measured as follows: nanoparticles (10 mg) were distributed in 3 mL of phosphate buffered saline (PBS, pH 7.4, 0.01 M) and then put into a dialysis membrane (MWCO, 8000 g/mol). Following this, this was introduced into 50 mL Falcon tube with 47 mL of PBS and then stirred at 100 rpm (37 °C). Whole media were exchanged at predetermined time intervals to prevent saturation of drug and then released drug was measured with a UV-spectrophotometer (UV-1601, Shimadzu Co. Ltd.) at 277 nm. The following expression was used for the calculations: