All statistical procedures were performed using Graphpad Prism 5.0 (version 5.0, San Diego, CA, USA). Statistically significant differences among more than two groups were analyzed using the one-way analysis of variance (ANOVA). Statistical significance was defined as * p < 0.05, ** p < 0.01, *** p < 0.001. For every experiment, three replicates were performed and all data were expressed as the mean ± SEM.
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