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Detection and quantification of Ascorbic Acid in nanovesicles were performed using a fluorometric Ascorbic Acid Assay Kit (Sigma-Aldrich, St. Louis, MO, USA). Samples were diluted in ascorbic acid buffer in a 96-well plate and subsequently to each well was added a catalyst and then reaction mix (the reaction mix is composed of an ascorbic acid buffer, ascorbic acid probe and ascorbic acid enzyme mix). After 5 min of incubation, fluorescence was read in a microplate reader at Ex/Em = 535/587 nm.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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