Chitosan was dissolved in an aqueous buffer solution of pH 4.5 (0.2 M HOAc/0.1 M NaOAc) to form a 1.5 mg/mL solution. The solution was divided into three portions that were kept at 4 °C, 20 °C and 50 °C for 42 days. Aliquots were taken from each portion every seven days and analyzed by GPC for MW determination.
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