4.9. siRNA-Based Gene Knockdown

For EGFR knockdown, cells were seeded at a 5x10⁴ cells/six-well concentration for 16 h before transfection. After 12 h, the transfection media was replaced with fresh media. Specific gene-targeting and control non-specific targeting small interfering ribonucleic acids (siRNAs) were obtained from Invitrogen (Thermo Fisher Scientific, Lafayette, CO, USA). siRNA targeting EGFR or a control sequence were transfected into cancer cells at a final concentration of 100 nM using Lipofectamine™ RNAiMAX Transfection Reagent. The siRNA target sequence for EGFR was 5′ -GGAUGUGAAGUGUGGCCAU-3′.