Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Focal cerebral ischemia-middle cerebral artery occlusion (MCAO) model

YY Yuan Yao
YJ Yingshi Ji
JR Jinghong Ren
HL Huanyu Liu
RK Rajesh Khanna
LS Li Sun
request Request a Protocol
ask Ask a question
Favorite

We used the middle cerebral artery occlusion (MCAO) method to induce 120 min of ischemia followed by 24 reperfusion [32]. Briefly, rats were anesthetized with chloral hydrate (0.4 ml/100 g, intraperitoneal injection). We then separated the external carotid artery (ECA) and internal carotid artery (ICA) along the common carotid artery (CCA), ligated the proximal end of the CCA and ECA, and prepared a thread at the distal end of the CCA. Then, we clamped the ICA with an arterial clip temporarily, made a small cut at 4 mm from the bifurcation of the CCA, inserted a nylon filament suture (Beijing Cinontech Co. Ltd. Beijing, China, 0.36 ± 0.02 mm) gently until it entered into the ICA, and then fixed the nylon filament with a thread around the distal end of the CCA. Rats were housed singly following the MCAO surgery.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A