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Flow cytometry analysis for lipid ROS
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Hepa1-6 cells were cultured in 6-well plates. Cells were treated and then 50 μM C11-BODIPY 581/591 (MX5211-1MG, MKBio, China) was added and incubated for 1 h. Lipid ROS generation was measured by a flow cytometer according to the method of Gao M, et al 39.
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