For the cell cycle assay, cells were collected and fixed in 75% PBS-diluted ethanol at 4 °C overnight. Then, after washing once with PBS, the cells were resuspended in 200 ul of BD Pharmingen PI/RNase staining buffer (BD Biosciences, #550825, USA) and incubated at room temperature for 15 min. We immediately ran the analysis on a flow cytometer (BD Biosciences, FACSCanto II, USA). The data were analyzed with ModFit software.
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