Step description

Specimens were collected in 60 different localities of continental Portugal. Fieldwork was carried out between 2015 and 2019.

Specimens were collected during fieldwork by direct search of specimens or by sweeping the vegetation with a hand-net and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).

Specimens were morphologically identified with the assistance of stereoscopic microscopes (Leica MZ12, 8x to 100x; Olympus SZX16, 7x to 115x) and using the available literature (Suppl. material 3). A subset (23%) was identified using the BOLD Identification Engine directly.

DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted and a 658 bp COI DNA barcode fragment was amplified and sequenced. For one specimen of Ceraleptus lividus, only a 418 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.

All obtained sequences were submitted to BOLD and GenBank databases and, to each sequenced specimen, the morphological identification, when available, was contrasted with the results of the BLAST of the newly-generated DNA barcodes in the BOLD Identification Engine.

Prior to submission to GBIF, data were checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).