Measurement of MMP:

Molt-4 cells were seeded in 6-wellplate and incubated with different concentrations of D-carvone (15 and 20 µM/ml) for 24 h. Rh-123 is a fluorescent probe used to estimate the depolarization of MMP. Rh-123 dye was added and incubated for 30 min at 37°C. After incubation the cells were washed with PBS and observed under fluorescence microscope (Labomed, USA). Fluorescence intensity of the captured images was analyzed by Image J software. Fluorescence intensity of the captured images was taken using a blue filter (450–490 nm).