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Propidium iodide uptake was measured by flow cytometry to assess cell death. After treatment, cells were stained with 1 μg·mL−1 PI (Invitrogen, Eugene, OR, USA) for 30 min at room temperature in the dark. PI uptake was quantified with a FACSCalibur Flow Cytometer (BD Biosciences, San Jose, CA, USA).
Copyright and License information: ©2021 The Authors. published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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