Cell viability assay

Jenna Giubilaro; Doris A. Schuetz; Tomasz M. Stepniewski; Yoon Namkung; Etienne Khoury; Mónica Lara-Márquez; Shirley Campbell; Alexandre Beautrait; Sylvain Armando; Olivier Radresa; Jean Duchaine; Nathalie Lamarche-Vane; Audrey Claing; Jana Selent; Michel Bouvier; Anne Marinier; Stéphane A. Laporte

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Cell viability assay

JG Jenna Giubilaro

DS Doris A. Schuetz

TS Tomasz M. Stepniewski

YN Yoon Namkung

EK Etienne Khoury

ML Mónica Lara-Márquez

SC Shirley Campbell

AB Alexandre Beautrait

SA Sylvain Armando

OR Olivier Radresa

JD Jean Duchaine

NL Nathalie Lamarche-Vane

AC Audrey Claing

JS Jana Selent

MB Michel Bouvier

AM Anne Marinier

SL Stéphane A. Laporte

This method is extracted from research article: Nat Commun, Aug 2021

Discovery of a dual Ras and ARF6 inhibitor from a GPCR endocytosis screen

DOI: 10.1038/s41467-021-24968-y

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MDA-MB-231 breast cancer cells and A549 lung carcinomas (1500 cells in 150 μl/well) were seeded overnight on a clear 96-well plate. Twenty-four hours later, the cells were treated with 0.1% DMSO, or different concentrations of Rasarfin (as indicated), or 10 µM of 21.4, 21.8 (MDA-MB-231 breast cancer cells) or Doxorubicin for the indicated times at 37 °C. At each time point, 10 μl of 5 mg/ml MTT was added to each well and the plates were incubated for an additional 4 h at 37 °C. The absorbance at 590 nm was measured by plate reader. Experiments were done in triplicate and repeated three times.

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