Wet lung tissue (100 mg) was homogenized with reagent provided with the MPO examination kit (Nanjing Jiancheng Bioengineering Institute) at a 1 : 19 ratio before boiling in water at 37°C for 15 min. Then, reagents were added and fixed according to the instruction manual. Ultimately, the samples were heated for 10 min in a 60° water bath, and absorbance was measured at 460 nm.
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