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For XTT assay, cells seeded in 24-well plates were incubated with TACS XTT labeling mixture (Trevigene). After 2 h supernatant of the cells were pipetted into 96-well plates and the conversion of XTT to formazan was quantified by measuring the spectral absorbance at 490 nm. Cell quantity was measured by seeding 2x104 cells in 6 cm2 plates and counted every 24 h via trypan blue exclusion.
Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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