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Cells were collected, cross-linked, and sonicated as described above and chromatin immunoprecipitation was performed following the protocol of the iDeal ChIP-seq kit for transcription factors (Diagenode). The immunoprecipitated DNA was used to perform qPCR with the SYBR Premix Ex Taq (Takara) and the Light Cycler 480 instrument (Roche). The relative amount of each amplified fragment was normalized with respect to the amplification obtained from input DNA. Primers were designed using the Primer3 web site94.
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