Sodium dodecyl sulfate polyacrylamide (SDS-PAGE) gel electrophoresis

HG Hui Xian Gan
HZ Hu Zhou
QL Qingsong Lin
YT Yen Wah Tong
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An aliquot of the samples was incubated for 0.5 h at room temperature in sample loading buffer containing 1% sodium dodecyl sulfate (SDS) and then analyzed by SDS-PAGE of 15% polyacrylamide gels with further staining by Coomassie brilliant blue G-250. The stained gels were scanned with G BOX gel doc system from Syngene and analyzed with the software of GeneTools to identify the protein size and amount.

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