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Multilocus sequence typing

SX Shu-zhen Xiao
SW Su Wang
WW Wen-man Wu
SZ Sheng-yuan Zhao
FG Fei-fei Gu
YN Yu-xing Ni
XG Xiao-kui Guo
JQ Jie-ming Qu
LH Li-zhong Han
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Multilocus sequence typing (MLST) was carried out as described previously (http://bigsdb.web.pasteur.fr/klebsiella/primers_used.html; Diancourt et al., 2005). Briefly, Seven hosekeeping genes (gapA, infB, mdh, pgi, phoE, rpoB, and tonB) for K. pneumoniae were amplified, sequenced, and analyzed. Alleles and STs were determined according to the database (http://bigsdb.web.pasteur.fr/perl/bigsdb/bigsdb.pl?db=pubmlst_klebsiella_seqdef_public&page=profiles). STs that could not be found in the database were submitted to the curator of the database (klebsiellaMLST@pasteur.fr). eBURST version 3.0 software was used to analyze the clustering of related STs. In this study, isolates were grouped together if six of the seven alleles were homologs.

Copyright and License information:  ©2017 Xiao, Wang, Wu, Zhao, Gu, Ni, Guo, Qu and Han.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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