CellTiter-Glo (CTG) cell viability assay

The CellTiter-Glo luminescent cell viability assay kit (Promega #G7573) was used to measure cell viability after drug treatment. The CellTiter-Glo kit determines the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. 500 to 1000 cells per well were seeded in a 96-well plate in 100 μL media volume. 24 hours later, cells were treated with indicated drugs by adding 100 μL medium containing 2x concentrated drug of desired final concentration. Cells were cultured in drug containing medium for 6 days, by then cell viability was determined by measuring the luminescent signal, following the manufacturer’s instructions. Survival fraction of drug-treated cells was normalized to DMSO-treated control. Survival, IC50 and statistics were determined by using the GraphPad Prism software.