Tail suspension test (TST)

A horizontal wooden bar having dimension 40 × 46 × 40 cm and about 35 cm above the ground was used for TST. Mouse was suspended by its tail from a wooden bar. Animal was secured to a bar by adhesive tape, which was placed 1-1.5 cm from the tail tip. This kept mouse head approximately 20 cm above the platform. Mouse exhibited escape-like behaviour immediately after suspension and subsequently showed immobility. A trial was performed for 5 min and the total immobility time of each animal was recorded.