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Having been vaccinated with 1/10 LD50 A. veronii (pN-PA-1) for 14 days, the challenge was conducted with 100 LD50 A. veronii C4 in the total amount of 4.98 × 107 CFU/g. Mortality was examined, and dead zebrafish were removed in subsequent 7 days. The relative percent survival (RPS) was determined according to the following formula. RPS=[1-(% mortality of immunized fish/% mortality of control fish)]× 100 (Byon et al., 2005).
Copyright and License information: ©2017 Liu, Huang, Hu, Tang, Ma, Yan, Han, Guo, Zhang, Sun and Liu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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