Thermal injury of the caudal tail fin

Thermal injury to the caudal fin was performed as previously described (Miskolci et al., 2019). Briefly, a fine tip (type E) of a line-powered thermal cautery instrument (Stoelting) was applied to the distal tip of the tail fin of tricaine-anesthetized larvae in the E3 medium for 1–2 s until the tail fin tissue curled but before injury to the notochord was observed. Following injury, the larvae were transferred to ZWEDGI devices or washed with E3 medium and allowed to recover at 28.5°C until live imaging or fixation at indicated times.