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GUS activity in transgenic leaf material was detected as described by Zhou et al. (2011). For SEM, tissue samples were fixed overnight in 3% v/v (volume/volume) glutaraldehyde in 25 mM phosphate buffer (pH 7.0), dehydrated with an ethanol series, then dried. SEM images were captured using a TM-3000 device (Hitachi) delivering an accelerating voltage of 15 kV.
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