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Hot plate experiments for thermal hyperalgesia testing

MH Maike Hartlehnert
AD Angelika Derksen
TH Tim Hagenacker
DK David Kindermann
MS Maria Schäfers
MP Mathias Pawlak
BK Bernd C. Kieseier
GH Gerd Meyer zu Horste
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Hot plate experiments were performed as previously described43 with minor modifications. Briefly, a small metal hot plate with a diameter of 1 cm (“7341 Plantar Test”; Ugo Basile) was heated to a constant temperature of 40 °C and was then lightly pressed onto the plantar surface of the hind paw of each experimental mouse. The time until paw withdrawal was recorded and defined as the thermal threshold (unit: seconds) and the experiment was repeated with the other hind paw. Both hind paws were measured three times per session with a recovery time of five minutes between measurements. Mean values of the technical replicates per session were calculated. All testing was performed by the same blinded investigator.

Copyright and License information: The Author(s) ©2017
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