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Following a single-round HIV-1 luciferase-reporter virus infection, culture media was aspirated and cells were washed with PBS. Cells were then lysed and processed using Firefly Luciferase HS Assay Kit (Genecopoeia, #LF009) according to the manufacturer’s protocol. Relative light units were measured by CentralPRO Microplate Luminometer (Berthold Technologies, #LB962).
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