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KT-MT attachment status analysis

RS Roshan L. Shrestha
AR Austin Rossi
DW Darawalee Wangsa
AH Ann K. Hogan
KZ Kimberly S. Zaldana
ES Evelyn Suva
YC Yang Jo Chung
CS Chelsea L. Sanders
SD Simone Difilippantonio
TK Tatiana S. Karpova
BK Baktiar Karim
DF Daniel R. Foltz
DF Daniele Fachinetti
PA Peter D. Aplan
TR Thomas Ried
MB Munira A. Basrai
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To define the KT-MT attachment status, we used NUF2 as the outer kinetochore marker and tubulin as the microtubule marker. We included only those cells in which more than 60% of KT-MT attachment status could be assigned. KT-MT attachments were assigned as end-on if the signals of tubulin in the microtubule bundles ended with NUF2. We categorized unattached and laterally attached kinetochores as non–end-on. KT-MT attachments were assigned as unattached if the NUF2 signal was free of tubulin signal, and laterally attached if NUF2 signal was on top of the tubulin signal of microtubule bundles.

Copyright and License information:  ©2021 Shrestha et al.
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