In Vitro Culturing of PDGFRα-GFP Bright and Dim iReFs

PDGFRα⁺-GFP^bright or -GFP^dim iReFs were isolated after 3 days of sham-operated or PNX mice and were sorted using a fluorescence-activated cell sorter, and then cultured for 24 and 72 hours with or without transforming growth factor (TGF)-β1. After this, the proliferation, activation, and differentiation status (Ki67⁺, CD34, CD29, and α-SMA) were analyzed using a fluorescence-activated cell sorter.