Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

M. massiliense Clone 1 isolates from the CO-RDP CF-NTM collection

RD Rebecca M. Davidson
JB Jeanne B. Benoit
SK Sara M. Kammlade
NH Nabeeh A. Hasan
LE L. Elaine Epperson
TS Terry Smith
SV Sruthi Vasireddy
BB Barbara A. Brown-Elliott
JN Jerry A. Nick
KO Kenneth N. Olivier
AZ Adrian M. Zelazny
CD Charles L. Daley
MS Michael Strong
RJ Richard J. Wallace, Jr
request Request a Protocol
ask Ask a question
Favorite

A total of 107 M. massiliense isolates from 64 patients were previously analyzed by WGS in a population genomics study by the CO-RDP9. Isolates were collected between 2013 and 2018, and patients were associated with CF facilities in 14 US states. Based on phylogenomic analyses, only 11 of 64 (17%) patients with M. massiliense had Clone 1 isolates in the previous study9. WGS data for a subset of 41 CO-RDP isolates, including Clone1 and non-Clone 1, were included in the current study (Table ​(Table1).1). Clone 1 isolates in the current study include 6 isolates from 6 patients (one isolate per patient) and two isolates per patient from three unique patients. Non-Clone 1 isolates in the current study include 21 isolates from 21 patients (one isolate per patient) and two isolates per patient from four unique patients.

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A