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2.3. Live Cell Confocal Microscopy

JS Joshua D. Simpson
DC Denni L. Currin-Ross
GE Gayathri R. Ediriweera
HS Horst Joachim Schirra
NF Nicholas L. Fletcher
CB Craig A. Bell
MA Maria C. Arno
KT Kristofer J. Thurecht
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Cells were imaged using a Zeiss 710 laser scanning confocal microscope (Carl Zeiss Microscopy, Oberkochen, Germany) housed within the Australian National Fabrication Facility (ANFF) Queensland Node. This machine is equipped with a heated stage, 40 × water objective, a 405-nm diode, and helium-neon and argon lasers. Prior to imaging, 5 × 105 cells in 1.5 mL of fresh medium were plated into slide-bottomed culture dishes (Mattek), returned to the incubator and left to grow overnight. The imaging conditions varied with the experimental applications as described below.

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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