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2.10. Kinetic Properties

CI Corinne Ivaldi
MD Mariane Daou
LV Laurent Vallon
AB Alexandra Bisotto
MH Mireille Haon
SG Sona Garajova
EB Emmanuel Bertrand
CF Craig B. Faulds
GS Giuliano Sciara
AJ Adrien Jacotot
CM Cyril Marchand
MH Mylène Hugoni
HR Harivony Rakotoarivonina
MR Marie-Noëlle Rosso
CR Caroline Rémond
PL Patricia Luis
ER Eric Record
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Kinetic parameters were determined in standard conditions with beechwood as substrate at concentrations of 1, 2, 5, 8, and 10 g/L. Experiments were performed in triplicate. Lineweaver–Burk plots were used to calculate the Michaelis constant KM and the enzyme turnover value kcat. The normalized equation v = (kcat/KM)[S]/(1 + [S]/KM) yielded the catalytic efficiency values (kcat/KM) with their corresponding standard errors.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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