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2.11. Tartrate-Resistant Acid Phosphatase Staining (TRAP Staining)
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TRAP staining was performed using the TRAP staining kit (Primary Cell, Sapporo, Japan) according to the manufacturer’s instructions. The activated multi-nucleated osteoclast cells on the bone resorption surface were counted using Image J software (NIH, Bethesda, MD, USA).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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