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Reference disc- and sample preparation

SB Steven V. Beekmans
KE Kaj S. Emanuel
TS Theodoor H. Smit
DI Davide Iannuzzi
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To verify proper functioning of our needle based indenter during in situ measurements, we tested a dummy IVD sample in which the NP was replaced with PDMS (Sylgard 184, Dow Corning) with a crosslinker:prepolymer weight ratio of 1:20. The PDMS was mixed, degassed for 30 min, poured into a glass petridish and allowed to cure at room temperature in a flow chamber for 96 hrs. Afterwards, the NP of an IVD was surgically removed by tweezers and a scalpel after drilling a small hole through the bone of the endplate. After NP removal, a cylinder of PDMS was cut from the cured sample and placed firmly into the disc. To fixate the PDMS in all directions the disc was resealed. The remainder of the PDMS in the petridish served as the polymeric reference sample.

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