ATP depletion and tracking

ATP levels were determined as indicated in the text using the BacTiterGlo kit (Promega). For ATP depletion assays, E. faecalis cells were grown to an OD₆₀₀ of ~0.25. Sodium arsenate dibasic heptahydrate (arsenate; Sigma-Aldrich) was added as indicated in the text to a final concentration of 10 mM, or an equivalent volume of water (solvent control) was added to the cells [56,57]. The cells were incubated for 30 min at 37°C, harvested by centrifugation at 2739xg, washed twice with 1x PBS, then resuspended in BHI prior to use for ATP determination. Colony forming units were determined via plating to conclude arsenate treatment did not result in cell death (S3 Fig). Relative light unit values (RLUs) yielded by the BacTiterGlo kit (Promega) were normalized to colony forming units taken at the time the ATP assay was performed. Minimum of n = 3 for all experiments.