2.7. Folin–Ciocalteu Total Phenol Content

To assess the molar concentration of the herbal extracts expressed as µM of gallic acid equivalent (GAE), 13 µL of each extract previously diluted 145 times was mixed for 20 s with 100 µL of Milli-Q water and 13 µL of Folin–Ciocalteu’s reagent in a well plate. After 5 min, 13 µL of a saturated sodium carbonate solution was manually added to each well, and absorbance was recorded at 765 nm after 2 h reaction. The GAEs were calculated through a gallic acid calibration curve (R² = 0.9995), and every sample was measured in triplicate.