Retinal Layer Segmentation

Raw three-dimensional SD-OCT images were exported to a numerical computing language (MATLAB; MathWorks, Natick, MA). The San Diego Automated Layer Segmentation Algorithm (SALSA) was used to automatically segment the RNFL layer.15 In brief, we assumed that each B-scan consists of several retinal layers (e.g., the Bruch's membrane layer, RNFL). Because the intraretinal layers have different thicknesses, each layer can be defined by a curve modeling its skeleton and a filter or set of filters modeling its thickness. To segment the different layers, it is sufficient to estimate their skeletons and the hyperparameters of the filters.