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Immunoblotting Analysis

BZ Biyun Zhu
HG Hua Gao
GX Gang Xu
DW Dewei Wu
SS Susheng Song
HJ Hongshan Jiang
SZ Shuifang Zhu
TQ Tiancong Qi
DX Daoxin Xie
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The total proteins were extracted from plants at 21 days after inoculation with mock or CMV2aTΔ2b. Fifty microgram of total protein for each sample was quantified and loaded for detection of coat protein (CP) of CMV2aTΔ2b. The antibody against coat protein (anti-CP) of CMV2aTΔ2b was produced by Abmart company (Abmart1) with the recombinant protein of the 1st to 154th AA of CP. The anti-CP was used as first antibody (1:6000), and anti-rabbit immunoglobulin antibody was used as the secondary antibody respectively (1:3000). All of the experiments were repeated at least three biological times.

Copyright and License information:  ©2017 Zhu, Gao, Xu, Wu, Song, Jiang, Zhu, Qi and Xie.
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