Staphylococcus aureus KCTC1621 (Gram+), and E. coli O157:H7 (Gram-) foodborne pathogens procured from the Korean Collection for Type Cultures (KCTC, Korea) were used in this study. For the growth and culture of strains, NB was used and cultures were incubated at 37°C, followed by maintenance of strains on nutrient agar (NA) slants at 4°C. To reactivate the cultures, cultures were taken out and a loop-full of colonies were inoculated in the fresh NB medium, and incubated for 24 h and 37oC. Further, sub-culturing was maintained in NB medium.
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